The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells

Abstract Background CSN5, a member of Cop9 signalosome, is essential for protein neddylation. It has been supposed to serve as an oncogene in some cancers. However, the role CSN5 not investigated cervical cancer yet. Methods Data from TCGA cohorts and GEO dataset was analyzed examine expression profile clinical relevance The on cell proliferation lines, Siha Hela, through knockdown via CRISPR–CAS9. Western blot used detect effect overexpression. biological behaviors were by CCK8, clone formation assay, 3-D spheroid generation assay cycle assay. Besides, vivo evaluated xenograft tumor model. MLN4924 given Hela with Results We found that downregulation cells inhibited vitro vivo, inhibitory effects largely rescued Moreover, deletion caused arrest rather than inducing apoptosis. Importantly, overexpression confers resistance anti-cancer (pevonedistat) cells. Conclusions Our findings demonstrated functions cancers may potential indicator predicting treatment future.